The vision of Life Science Leader is to be an essential business tool for life science executives. Our content is designed to not only inform readers of best practices, but motivate them to implement those best practices in their own businesses.
Issue link: https://lifescienceleadermag.epubxp.com/i/161331
BIO INNOVATION NOTES Innovation In Expression Systems Yields Increased Productivity By Eric Langer, president and managing partner, BioPlan Associates, Inc. T echnological advances in genetic engineering, particularly expression systems (the genetically modified cells that express desired proteins), process design, and equipment continue to be combined such that the same amount of drug product can be manufactured at a much smaller scale. Today, smaller-scale, less-expensive equipment is permitting more rapid drug development and production in smaller facilities. This year's 10th Annual Report and Survey of Biopharmaceutical Manufacturing Capacity and Production continues to show overall increased productivity and efficiency in biomanufacturing. The average expression yield (amount of protein produced in a fixed bioreactor fluid volume), exemplified by mammalian cell culture production of monoclonal antibodies, is now reported to be 2.68 grams/Liter for late-stage clinical supplies manufacturing and 2.29 grams/Liter for commercial-scale manufacturing. These production yields have been increasing since 2008 at an average annual growth rate of 9.8 percent. This almost exclusively involves cell culture using Chinese hamster ovary (CHO) cells. With CHO serving very well and major changes generally avoided in this highly regulated industry, adoption of other and improved expression systems, despite offering further advantages, remains slow. Because bioprocessing for drug products is generally "lockeddown" early in development, the reported clinical supply yields more reflect the current state-of-the-art for new bioprocesses. It must be kept in mind that, at any time, the great majority of commercial-scale manufacturing involves established products with their original bioprocessing usually developed decades ago, with these facilities operating at much lower titres than now reported for new processes. Only about a decade ago, expression yields of up to 1 gram/ Liter were considered truly state-of-the-art. Many older products, including many current blockbuster (≥$1 billion/year sales) monoclonal antibody products, such as those originally developed in the 1980s, were developed with yields of only a few tenths gram/Liter. This generally required multiple≥10,000 L bioreactors operating continuously for commercial manufacture. Many of the largest biopharmaceutical facilities now have excess capacity, with legacy facilities either being taken offline, refurbished, or used to provide CMO services, manufacturing products for other companies. About 3 grams/L yield could soon become the future reference standard for monoclonal antibody and other mammalian 20 LifeScienceLeader.com cell-based manufacture, with further steady growth likely. At 3 grams/L, a single-use 2,000 L bioreactor production run can provide ~6,000 g or 6 kg of protein, with even a low-potency, cheaper product providing sales approaching $100 million. If larger amounts of product are needed to support sales, multiple single-use process lines can be run in parallel. For comparison, a yield of 0.6 gram/L would require 10,000 L capacity, e.g. a 10,000 L stainless steel bioreactor to manufacture the same amount of product. Operating at higher yield and lower scale to produce the same amount of product provides savings in many areas, including culture media and other supplies, space, utilities, infrastructure/support, and labor. Further, during the past decade, most bioprocessing at precommercial scales is now being performed using single-use (dispose of after use) plastics-based rather than fixed stainless steel equipment. Our study reports about 2/3 of all new processes are being developed using single-use systems. And as products in the development pipeline manufactured using single-use systems gain approvals, commercial manufacturing will increasingly be performed using single-use systems and at much smaller scales than conventional stainless steel facilities. Attaining even higher yields in mammalian systems at large scale, such as ≥5 grams/L, is becoming much more attainable. Some CMOs are now offering routine monoclonal antibody scale-up at high yields. For example, the PER.C6 cell line development joint venture of DSM and Crucell (now the Center for Vaccines, Johnson & Johnson Co.) has reported a record level titre of over 30 grams/L harvest for an antibody product. Presuming yields of just 10 grams/L will be attainable in practice at commercial scale in coming years, a 1,000 L bioreactor will be able to match the output of four 2,000 L bioreactors operating at the current state-of-the-art of about 2.5 grams/L or a 10,000 L bioreactor operating at 1 gram/L. CONTINUOUS PROCESSING IMPROVEMENTS ON THE RISE Besides improvements in genetic engineering of cell lines and adoption of single-use systems, the industry is seeing rapid adoption of perfusion, using pumps and filters or other methods to increase the concentration of cells and expressed products within bioreactors, with this further increasing productivity. Other improvements contributing to increased expression yields include improved culture media and process design, with both culture media selection and bioprocessing design September 2013